ARG40420
anti-TCF21 antibody
anti-TCF21 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TCF21 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog, Gpig, Hrs, Rb |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TCF21 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminal region of Human TCF21 (within the following region: SNCENGSPQKGRGGLGKRRKAPTKKSPLSGVSQEGKQVQRNAANARERAR). |
| Conjugation | Un-conjugated |
| Alternate Names | Class A basic helix-loop-helix protein 23; TCF-21; Epicardin; Pod-1; bHLHa23; Transcription factor 21; Capsulin; Podocyte-expressed 1; POD1 |
Application Instructions
| Predict Reactivity Note | Predicted Homology Based On Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Rabbit: 100% | ||||||
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| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HeLa and Human lung |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| Preservative | 0.09% (w/v) Sodium azide |
| Stabilizer | 2% Sucrose |
| Concentration | Batch dependent: 0.5 - 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TCF21 |
| Gene Full Name | transcription factor 21 |
| Background | TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 20 kDa |
Images (3) Click the Picture to Zoom In
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ARG40420 anti-TCF21 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain, cortex, senile plaque tissue stained with ARG40420 anti-TCF21 antibody at 5 µg/ml dilution. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
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ARG40420 anti-TCF21 antibody WB image
Western blot: HeLa whole cell lysate stained with ARG40420 anti-TCF21 antibody at 1 µg/ml dilution.
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ARG40420 anti-TCF21 antibody WB image
Western blot: Human lung tissue lysate stained with ARG40420 anti-TCF21 antibody at 0.2 - 1 µg/ml dilution.
