ARG66922
anti-TIM3 antibody [SQab22265]
anti-TIM3 antibody [SQab22265] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab22265] recognizes TIM3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab22265 |
| Isotype | IgG |
| Target Name | TIM3 |
| Antigen Species | Human |
| Immunogen | A synthetic peptide corresponding to the sequence of Human TIM-3. |
| Conjugation | Un-conjugated |
| Protein Full Name | Hepatitis A virus cellular receptor 2 |
| Alternate Names | KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05%BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8TDQ0 Human Hepatitis A virus cellular receptor 2 |
|---|---|
| Gene Symbol | HAVCR2 |
| Gene Full Name | hepatitis A virus cellular receptor 2 |
| Background | The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011] |
| Function | Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related news: New PD-1 ELISA Kit, excellent for preclinical studies or pharmatheutical development |
| Research Area | Immune System antibody |
| Calculated MW | 33 kDa |
| PTM | O-glycosylated with core 1 or possibly core 8 glycans. Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9. |
Images (3) Click the Picture to Zoom In
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ARG66922 anti-TIM3 antibody [SQab22265] WB image (Customer's Feedback)
Western blot: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:2000 dilution for 1 hour at room temperature.
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ARG66922 anti-TIM3 antibody [SQab22265] FACS image (Customer's Feedback)
Flow Cytometry: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:200 dilution for 50 min at 4ºC.
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ARG66922 anti-TIM3 antibody [SQab22265] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil stained with ARG66922 anti-TIM3 antibody [SQab22265]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).
Customer's Feedback
Excellent
Review for anti-TIM3 antibody [SQab22265]
Application：WB
Sample：NK92-MI; TIM3 KD NK92-MI; Primary T cell
Sample Loading Amount：35 μg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：1 h
Primary Antibody Incubation Temperature：Room Temperature ºC
Excellent
Review for anti-TIM3 antibody [SQab22265]
Application：FACS
Sample：Primary T cell; NK92-MI; TIM3 KD NK92-MI
Cell Number/Concentration：5x10^5 cells
Fixation Buffer：-
Fixation Time：-
Fixation Temperature：- ºC
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：50 min
Primary Antibody Incubation Temperature：4 ºC
