ARG23092
anti-TLN1 / Talin 1 antibody [TA205]
anti-TLN1 / Talin 1 antibody [TA205] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Chicken,Rabbit
Overview
| Product Description | Mouse Monoclonal antibody [TA205] recognizes TLN1 / Talin 1 Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a ~225 kDa cytoskeletal protein. Mouse anti Human Talin antibody, clone TA205 may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. Mouse anti Human Talin antibody, clone recognizes an epitope between residues 139 and 433. |
|---|---|
| Tested Reactivity | Hu, Chk, Rb |
| Tested Application | FACS, ICC/IF, IHC-Fr, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TA205 |
| Isotype | IgG1 |
| Target Name | TLN1 / Talin 1 |
| Antigen Species | Human |
| Immunogen | Human platelet talin. |
| Conjugation | Un-conjugated |
| Alternate Names | Talin-1; TLN; ILWEQ |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Membrane permeabilization is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TLN1 |
| Gene Full Name | talin 1 |
| Background | This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009] |
| Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity). [UniProt] |
| Calculated MW | 270 kDa |
Images (1) Click the Picture to Zoom In
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ARG23092 anti-TLN1 / Talin 1 antibody [TA205] FACS image
Flow Cytometry: Human peripheral blood platelets stained with ARG23092 anti-TLN1 / Talin 1 antibody [TA205].
