ARG56919
anti-TLR7 antibody [4F4]
anti-TLR7 antibody [4F4] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [4F4] recognizes TLR7 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4F4 |
| Isotype | IgG1, kappa |
| Target Name | TLR7 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 451-500 of Human TLR7. |
| Conjugation | Un-conjugated |
| Alternate Names | TLR7-like; Toll-like receptor 7 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TLR7 |
| Gene Full Name | toll-like receptor 7 |
| Background | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008] |
| Function | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). [UniProt] |
| Calculated MW | 121 kDa |
Images (3) Click the Picture to Zoom In
-
ARG56919 anti-TLR7 antibody [4F4] ICC/IF image
Immunoflorescense: THP-1 cell line stained with ARG56919 anti-TLR7 antibody [4F4] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
-
ARG56919 anti-TLR7 antibody [4F4] FACS image
Flow Cytometry: THP-1 cell line stained with ARG56919 anti-TLR7 antibody [4F4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
-
ARG56919 anti-TLR7 antibody [4F4] WB image
Western blot: 80 µg of Ramos cell lysates stained with ARG56919 anti-TLR7 antibody [4F4] at 1:1000. Arrow indicates TLR7 protein in Human Ramos cells.
