ARG55738
anti-ZO1 antibody
anti-ZO1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Dog,Human,Monkey,Mouse,Rat
Publication8
Overview
| Product Description | Goat Polyclonal antibody recognizes ZO1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Mk |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ZO1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant peptide around aa. 1580 (C-terminus) of Human ZO-1 |
| Conjugation | Un-conjugated |
| Alternate Names | Zonula occludens protein 1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; ZO-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TJP1 |
| Gene Full Name | tight junction protein 1 |
| Background | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Function | The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. [UniProt] |
| Calculated MW | 195 kDa |
| PTM | Phosphorylated. Dephosphorylated by PTPRJ. |
Images (2) Click the Picture to Zoom In
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ARG55738 anti-ZO1 antibody WB image
Western blot: 50 µg of total protein (MDCK cell lysate) stained with ARG55738 anti-ZO1 antibody at 1:2500 dilution.
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ARG55738 anti-ZO1 antibody ICC/IF image
Immunocytochemistry: primary RPE cells stained with ARG55738 anti-ZO1 antibody at 1:100 dilution.
Specific References
Intestinal epithelium-specific Fut2 deficiency promotes colorectal cancer through down-regulating fucosylation of MCAM
IHC-P / Mouse
Incomplete Recovery from the Radiocontrast-Induced Dysregulated Cell Cycle, Adhesion, and Fibrogenesis in Renal Tubular Cells after Radiocontrast (Iohexol) Removal
WB / Human
MicroRNA-29b Suppresses Inflammation and Protects Blood-Brain Barrier Integrity in Ischemic Stroke
WB / Mouse
Long non-coding RNA H19 promotes leukocyte inflammation in ischemic stroke by targeting the miR-29b/C1QTNF6 axis
WB / Rat
Gut microbiota-mediated lysophosphatidylcholine generation promotes colitis in intestinal epithelium-specific Fut2 deficiency.
WB / Mouse
Probiotic mixtures with aerobic constituent promoted the recovery of multi-barriers in DSS-induced chronic colitis.
WB / Mouse
Catalpol provides a protective effect on fibrillary Aβ1-42 -induced barrierdisruption in an in vitro model of the blood-brain barrier.
WB / Rat
Hyperoside protects the blood-brain barrier from neurotoxicity of amyloid beta 1-42.
WB / Mouse
