ARG67027
anti-alpha Fetoprotein antibody [SQab30312]
anti-alpha Fetoprotein antibody [SQab30312] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
1
Overview
| Product Description | Recombinant rabbit Monoclonal antibody [SQab30312] recognizes alpha Fetoprotein |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab30312 |
| Isotype | IgG |
| Target Name | alpha Fetoprotein |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human alpha Fetoprotein. |
| Conjugation | Un-conjugated |
| Protein Full Name | Alpha-fetoprotein |
| Alternate Names | AFP, Alpha Fetoprotein, Alpha-Fetoprotein, HPAFP , FETA, Alpha-1-Fetoprotein, Alpha-Fetoglobulin, AFPD |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Hepatocellular carcinoma | ||||||
| Observed Size | 65-72 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05%BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AFP |
| Gene Full Name | Alpha Fetoprotein |
| Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019] |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [Uniprot] |
| Cellular Localization | Secreted |
| Calculated MW | 69 kDa |
| PTM | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG67027 anti-alpha Fetoprotein antibody [SQab30312] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded hepatocellular carcinoma stained with ARG67027 anti-alpha Fetoprotein antibody [SQab30312].
Customer's Feedback
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Review for anti-alpha Fetoprotein antibody [SQab30312]
Application：WB
Sample：HepG2
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
