ARG51814
anti-eIF4B phospho (Ser422) antibody
anti-eIF4B phospho (Ser422) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eIF4B phospho (Ser422) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eIF4B |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B. |
| Conjugation | Un-conjugated |
| Alternate Names | EIF-4B; eIF-4B; PRO1843; Eukaryotic translation initiation factor 4B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P23588 Human Eukaryotic translation initiation factor 4B Swiss-port # Q8BGD9 Mouse Eukaryotic translation initiation factor 4B |
|---|---|
| Gene Symbol | EIF4B |
| Gene Full Name | eukaryotic translation initiation factor 4B |
| Background | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 69 kDa |
| PTM | Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. |
Images (2) Click the Picture to Zoom In
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ARG51814 anti-eIF4B phospho (Ser422) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with EGF stained with ARG51814 anti-eIF4B phospho (Ser422) antibody.
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ARG51814 anti-eIF4B phospho (Ser422) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung carcinomatissue stained with ARG51814 anti-eIF4B phospho (Ser422) antibody (left) or the same antibody preincubated with blocking peptide (right).
