ARG30110

Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB)

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication4

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG20006 anti-Cytochrome C antibody Rabbit pAb Bov, Chk, Dog, Hu, Ms, Rat IP, WB 25 μg
ARG20048 anti-Caspase 9 (cleaved) antibody Rabbit pAb Hu IP, WB 25 μg
ARG20001 anti-Caspase 3 antibody [C33] Mouse mAb Hu WB 25 μg
ARG20041 anti-PARP (cleaved) antibody Rabbit pAb Hu IHC, WB 25 μg
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl
ARG65350 Goat anti-Mouse IgG antibody (HRP) Goat pAb Ms ELISA, IHC-P, WB 50 μl

Overview

Product Description

Apoptosis is the process of programmed cell death that may occur in multicellular organism. Characteristic phenomena of apoptosis includes surface membrane blebbing, cell shrinkage, nuclear and cytoplasmic condensation, and DNA fragmentation.
This Mitochondria/Caspase-dependent Apoptosis kit investigates the apoptosis process via downstream pathway of Cytochrome C released from mitochondria. The Bcl-2 family proteins regulate apoptosis by controlling mitochondrial permeability. The anti-apoptotic proteins Bcl-2 and Bcl-xL reside in the outer membrane of mitochondria and inhibit cytochrome c release. The apoptotic proteins Bad, Bid, Bax, and Bim, otherwise, promote Cytochrome C release and trigger cell death. Upon release from mitochondria, cytochrome c binds to Apaf-1 and forms apoptosome with Caspase-9. Subsequently, proCaspase-3 will be cleaved and activated. Finally, PARP is inactivated by active caspase-3 cleavage and cell death occurs.

Target Name Mitochondria/Caspase dependant Apoptosis
Alternate Names Mitochondria/Caspase dependant Apoptosis antibody; Caspase 3 antibody; Cytochrome C antibody; PARP (cleaved) antibody; Caspase 9 (cleaved) antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for Mitochondria/Caspase dependant Apoptosis (Caspase3, Caspase9, Cytochrome c, PARP)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Specific References

KDF1 Promoted Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells through Activating STAT3 and AKT Pathway

ARG65350: WB / 

Yi-Qing Guo et al.
Biomedicines.,  (2023)

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Single-Cell Analysis of Signaling Proteins Provides Insights into Proapoptotic Properties of Anticancer Drugs.

ARG20006: scPISA / Human

Yanrong Wen et al.
Anal Chem.,  (2020)

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Probing apoptosis signaling proteins in single living cells for precision efficacy evaluation of anti-cancer drugs.

ARG20006: / Human

Wen Yanrong et al.
Preprint.,  (2019)

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Inhibition of histone acetylation by curcumin reduces alcohol-induced fetal cardiac apoptosis.

ARG20001: WB / Mouse

Yan X et al.
J Biomed Sci.,  (2017)

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