ARG43961

anti-NAAA antibody

anti-NAAA antibody for ELISA,Flow cytometry,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NAAA
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NAAA
Antigen Species Human
Immunogen Human NAAA recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name N-acylethanolamine-hydrolyzing acid amidase
Alternate Names NAAA; N-Acylethanolamine Acid Amidase; ASAHL; N-Acylethanolamine-Hydrolyzing Acid Amidase; Acylsphingosine Deacylase NAAA; Acid Ceramidase-Like Protein; ASAH-Like Protein; PLT; N-Acylsphingosine Amidohydrolase (Acid Ceramidase)-Like; N-Acylsphingosine Amidohydrolase-Like; EC 3.5.1.60; EC 3.5.1.23

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg /1x10^6 cells
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27163 Human NAAA

GeneID: 497009 Rat NAAA

GeneID: 67111 Mouse NAAA

Gene Symbol NAAA
Gene Full Name N-Acylethanolamine Acid Amidase
Background Enables N-(long-chain-acyl)ethanolamine deacylase activity; N-acylsphingosine amidohydrolase activity; and fatty acid amide hydrolase activity. Involved in several processes, including N-acylethanolamine metabolic process; N-acylphosphatidylethanolamine metabolic process; and sphingosine metabolic process. Located in lysosome. Is extrinsic component of membrane.
Function Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine.
Cellular Localization Lysosome, Membrane
Calculated MW 40 kDa
PTM Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Images (4) Click the Picture to Zoom In

  • ARG43961 anti-NAAA antibody WB image

    Western blot: 293T stained with ARG43961 anti-NAAA antibody at 0.5 μg/mL dilution.

  • ARG43961 anti-NAAA antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG43961 anti-NAAA antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43961 anti-NAAA antibody WB image

    Western blot: Rat liver stained with ARG43961 anti-NAAA antibody at 0.5 μg/mL dilution.

  • ARG43961 anti-NAAA antibody WB image

    Western blot: Mouse liver stained with ARG43961 anti-NAAA antibody at 0.5 μg/mL dilution.